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We show that KC and RHE are more rigid than EPI and FRS, which are more rigid than SC. Also, we demonstrate that a greater thickness of the SC layers is correlated with a less flexible and more hard SC layer. We further demonstrate that the SC layers of in vivo and EPIs have mechanical properties similar to that of intact SC, whereas that of KC has mechanical properties similar to that of mature EPIs. These results demonstrate that our cell-culture-based models are suitable surrogates to study SC properties. UNPUBLISHED UNITED STATES COURT OF APPEALS FOR THE FOURTH CIRCUIT No. 18-7748 ORLANDO JOHNSON, Plaintiff - Appellant, v. PAMELA L. CHARLES, Former Warden of Food and Agriculture; HARRELL WATTS, Administrative Director of the SCDC; BONITA R. JOHNSON, Superintendent of SCDC; RICKIE ANDERS, Warden of the Greenville Correctional Center; MARK LEWIS, Disciplinary Hearing Officer; System Requirements: Mac OS X 10.7 or later and 512 MB RAM. Mac OS X 10.8 or later and 1GB RAM. 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